AbstractOne major bottleneck in correlative microscopies lies in the final image registration step. Accurate localization of the signal of interest from the primary imaging modality, into the subsequent imaging modalities, with error estimation is key to assign a function to a structure. To conduct this work efficiently and give an easy access to all scientists, we designed eC-CLEM in the Icy BioImageAnalysis platform with a wide range of applications including registration error estimation. We will introduce this tool and explore the application range of it.
Dr Xavier Heiligenstein
Chief Scientific Officer CryoCapCell
Xavier Heiligenstein obtained his Master in cell biology, physiology and pathologies at the university Denis Diderot Paris 7 before completing a PhD at the EMBL in Cell Biology and biophysics in 2011, under the supervision of Claude Antony. While establishing a Correlative Light and Electron Microscopy workflow to study the ultrastructure of the Xenopus laevis mitotic spindle by large scale electron tomography (HPF-FS, serial section, montage tomography), he developed a novel technology, the CryoCapsule.
He was hired at Institut Curie in 2012 for a post-doc to adapt the CryoCapsule to fast processes analysis by CLEM such as endosome dynamics in the team of Graça Raposo. There, several other technological limitation were identified: after co-developing eC-CLEM together with Perrine Paul-Gilloteaux, he supervised the creation, development and application of the HPM Live-µ by the company he co-founded: CryoCapCell.
More recently, he worked on a novel embedding resin the R221, which reduces electron charging in Scanning Electron Microscope while preserving bio-fluorescence to expand volume correlative microscopy of vitrified samples.