AbstractNudiviruses are large dsDNA rod-shaped enveloped viruses infecting a wide range of hosts. The best characterized member of the family, Oryctes Nudivirus (OrNV), infects rhinoceros beetle, a devastating pest damaging coconut and oil palm trees in Southeast Asia and the Pacific islands. Although previous electron microscopic studies have established the have established the timeline for virus entry, replication and budding, little is known regarding the mechanism of viral assembly and egress. In this study we used volume microscopy to characterise the membranous and cellular changes incurred during nucleocytoplasmic transport and viral egress following OrNV infection. OrNV appears to assemble into a mature particle inside the nuclear compartment and to cross the nuclear envelope using a series of membrane vesicles, to transit through the cytoplasm inside some vesicular bodies, before being released into the extracellular space either as single virions or a as virions contained inside vesicles. This study emphasis on the power of volume microscopy in obtaining detailed structural information on viral replication using OrNV as a model.
University or Otago
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