Volume Imaging with Electrons


Keynote – Technique in Focus – Vendor Session

Chat Transcript

Please add your questions to the chat to be addressed at the end of the presentation.
00:26:16 Rick Webb
Doesn't the 3rd rule of big data club ("automate analysis and throw away the data") assume that future analysis approaches will be based on current approaches? New algorithms for machine learning are being developed all the time, and the input data for one method isn't always compatible with another. How do you ensure you know which data to throw away?
00:42:58 Benjamin Padman
do the volunteers get to see the data from multiple directions? One thing that is difficult is where you first start to hit a structure. It just looks like a blob.
00:47:36 Jeremy Shaw
What is the uptake for the EMPIAR public image archive? Is this promoted and encouraged at the facility level? Or is this part of the researchers data storage/archive plan?
00:49:24 Minh Huynh
You spend so much effort training the neural network, will others be able to use the trained models for their own data?
00:52:07 Gediminas Gervinskas
as long as the experiment can be repeated
00:55:04 Jeremy Shaw
Thanks everyone, have a great meeting!
00:59:11 Lucy Collinson
Thank you Lucy!
00:59:20 Minh Huynh
Please add your questions to the chat to be addressed at the end of the presentation.
01:09:53 Minh Huynh
Thanks Minh
01:10:31 Sue Lindsay
How often is zero-loss energy filtered imaging you mentioned used in cryo-EM tomography and are inelastic images used at all (or is the data just too noisy)?
01:12:13 Timothy Petersen
Can you please explain a bit more about the stress relief cuts that you use to help prevent bending.
01:27:45 Ashalatha I K
Do you think that slice-and-view imaging and lamellar preparation could be combined into a single workflow? i.e. mill and image until you reach the region you're interested in, then use that to define the lamella location?
01:30:04 Benjamin Padman
Would an environmental glove box (controlled temperature and humidity) be beneficial in the transfer of cells from their culture wells to the vitrobot?
01:30:13 Filip Braet
@Georg could you please send a link to the paper re stress relief cuts?
01:31:56 Minh Huynh
great talk Georg!
01:34:09 Filip Braet
Hi Minh, here the paper https://www.sciencedirect.com/science/article/pii/S104784771930200X
01:35:53 Georg Ramm
Many thanks Georg and thank you for a great talk!
01:36:25 Minh Huynh
Please add you questions to the chat to be addressed at the end of both vendor presentations.
01:42:48 Minh Huynh
Given that you've gone to the trouble of installing the laser optics in the PFIB, can the system also collect photons that bounce back? Can we do fluorescence imaging with it?
01:51:56 Benjamin Padman
maybe even cathodoluminescence?
01:52:45 Benjamin Padman
Here's a related example for atom probe tomography:
01:54:56 Timothy Petersen
01:54:58 Timothy Petersen

In this Session

Keynote Session

(25min followed by 10min live Q&A)

Lucy Collinson The Francis Crick Institute
Towards Quantitative Correlative Microscopy


Technique in Focus

(30min followed by 5min live Q&A)

Vendor Session

(2 x 10min followed by 5min live Q&A)

David Stowe Product Manager, Gatan Inc.
Whole-cell imaging using 3View2.XP serial block-face scanning electron microscopy

Brandon Van Leer Thermo-Fisher Scientific
Helios 5 Laser PFIB for Materials Science