I didn't know these interesting facts about my own body.
00:09:48 Timothy Petersen
How thick can a routine sample be, and can we do frozen samples (to preserve diffusible elements)?
00:16:51 Peta Clode
(I don't actually speak this slowly)
00:17:41 Nik Tatarnic (WA Museum)
Great question, Peta - no simple answer. We can easily /mount/ a specimen of 4 mm diameter (the rice grain, for example), but the real issue is probably the self absorption. In this case, the question is rephrased as: "what thickness of the specimen matrix will attenuate (eg) 30% of the fluorescence of the element that you are interested in?"
00:20:03 Martin de Jonge
-- LMK if that makes sense.
00:20:10 Martin de Jonge
How did you preserve the Sarawak bugs in the field for microCT?
00:20:53 Benjamin Padman
00:22:21 Nik Tatarnic (WA Museum)
00:22:24 Nik Tatarnic (WA Museum)
Can't wait to come visit the WA museum! Looks Amazing.
00:22:47 Crystal Cooper
Bugs were stored in 100% ethanol, mainly for the dna
00:22:51 Nik Tatarnic (WA Museum)
Didn't seem to affect internal morphology much
00:23:05 Nik Tatarnic (WA Museum)
00:23:16 Benjamin Padman
@Nik - do you put it straight into 100% or do an ethanol series?
00:23:33 Minh Huynh
oh; the other part was about cryo presentation. We can do the measurement within a flow of cryogenic N2 gas, and have done this on several specimens. So: yes, cryo tomography is possible. To be fair, we do have some small issues getting cryo specimens into the beam, but find that the easiest option has been to freeze the specimen in situ, in the cryostream.
00:23:35 Martin de Jonge
Fabulous presentation! Were the troglofauna models hand-painted too? They look translucent with colour through the interior.
00:24:36 Adrian Sheppard
I think you have the best job Nik!
00:24:37 Minh Huynh
Cool stuff Nik!
00:24:41 Peta Clode
I was in the middle of the rainforest with limited resources, so straight into ethanol.
00:24:54 Nik Tatarnic (WA Museum)
Fair enough! 🙂
00:25:22 Minh Huynh
Am loving these 3D images and am very jealous of your job Nik.
00:25:44 Sarah Ellis
[Peta] We would love to work with you; feel free to contact us about doing your measurements. There is some work with Antony van der Ent in the literature that show the kinds of things that we can do for plant imaging.
00:26:00 Martin de Jonge
That was so cool, Nikolai! Do you work at all with Aboriginal TOs/ corporations imaging any of their archaeological artefacts from significant site across Australia?
00:27:54 Justyna Miszkiewicz
00:28:06 Justyna Miszkiewicz
Hi Justyna, not sure about Nik, but we have done a number of aboriginal artefacts at the CMCA.
00:29:28 Jeremy Shaw
At the moment there are no formal plans, but I'm hoping that the Mandu Mandu work has shown our culture and communities people the potential of this technology.
00:29:30 Nik Tatarnic (WA Museum)
Great talk Nik! At ANU our ARC ITTC has 'Heritage' theme, which includes work on museum collections https://m3d.edu.au/research.html .We are interested in developing this more 🙂
00:29:57 levi beeching
Thanks Levi. There's great potential here!
00:31:08 Nik Tatarnic (WA Museum)
That's fantastic, Nik and Jeremy. Agreed, there's so much potential particularly that these are non-invasive methods. Levi - Tim Denham in my School (I'm in ANU RSHA) has spoken a lot about the ITTC, it sounds like an amazing initiative!
00:35:24 Justyna Miszkiewicz
Hi Jaesung, any plans of doing this in vivo using something like Exitron 12000 contrast agent and ofcourse using an in vivo CT?!
00:36:52 Diana Patalwala
Hi Diana, I would like to try live imaging but we do not have to facility in house and there's logistic challenges to to take out and bring back to our animal facility.
00:39:05 Jaesung Choi
Okayy fair enough!
00:41:28 Diana Patalwala
Jaesung, sorry my head was turned, I would like more detail on how you did the targeted staining of your lesions, did you say a platinum based drug?
00:41:42 Jeremy Shaw
We used contrast staining (Osmium Tetraoxide or Lugol's Iodine Staining) to distinguish brain tissue (high lipid content) vs blood filled or empty blood vessels.
00:43:10 Jaesung Choi
Brite Vu is something else you could try as opposed to Microfil, is much easier to work with as well!
00:44:47 Diana Patalwala
Thanks Diana, we will have a look as well 🙂
00:46:14 Jaesung Choi
maybe for brain, but there are a number projects at UWA that use resin perfusion for visualising vasculature. Placental mainly
00:55:53 Jeremy Shaw
00:56:18 Jeremy Shaw
Thanks Jeremy, I will have a look at that as well
00:57:42 Jaesung Choi
cant wait 🙂
00:59:17 Jeremy Shaw