Volume Imaging with X-Rays

SESSION 4

Problem Solver Session

Chat Transcript

Welcome everyone. Don't forget to submit you questions into the chat.
00:09:01 Levi Beeching (CTLab, ANU)
Why such a big rebound? Is this cells breaking down?
00:19:18 Levi Beeching (CTLab, ANU)
I was thinking this also, wouldn't expect such a large change/rebound
00:20:03 Jeremy Shaw
maybe something related to dramatic shrinkage early on
00:21:43 Jeremy Shaw
How large a specimen do you think can be effectively stained with iodine/Lugols before you have trouble with penetrating the deepest parts?
00:21:46 Sheridan Mayo
sherry, even large samples such as alligator heads. It just takes far longer. Concentration also important
00:23:07 Jeremy Shaw
Is there a rule of thumb regards concentration and subject depth?
00:23:55 Levi Beeching (CTLab, ANU)
high conc normally = high shrinkage
00:24:35 Jeremy Shaw
but in general it's not particularly well quantified in the literature
00:25:15 Jeremy Shaw
Magnificant datasets!
00:25:29 Sheridan Mayo
Hi Jeremy, are some of the stains more compatible with EM imaging than others, if you want to do correlative imaging?
00:26:26 Adrian Sheppard
@Adrian I’ve used osmium to do X-ray and SBF SEM correlation but of course that’s standard for EM so it works fine.
00:29:27 Minh Huynh
Is the iodine likely to penetrate better as an ethanol solution?
00:29:48 Rick Webb

In this Session

Problem Solver Session

(20min followed by 5min live group Q&A)

Jeremy Shaw CMCA, The University of Western Australia
Staining approaches to imaging biology with X-ray micro-CT